Membrane antigen on Epstein-Barr virus-infected human B cells recognized by a monoclonal antibody Academic Article Article uri icon

Overview

MeSH Major

  • Antineoplastic Agents
  • Bone Neoplasms
  • Radium

abstract

  • This paper describes a monoclonal antibody (B532) that detects a membrane antigen present on greater than or equal to 95% of the B cells from lines carrying the Epstein-Barr virus (EBV) genome. Evidence suggesting that B532 is EBV-related was originally obtained by using a cell-binding radioassay with different cell line substrates. Immunofluorescence and cell-sorter analysis confirmed that the antigen was present in high density on all EBV-infected lymphoblastoid B-cell lines, but not on EBV-negative B-, T-, myeloid, or null cell lines. Isolated normal peripheral blood B and T lymphocytes and monocytes failed to bind B532. The monoclonal antibody did not inhibit in vitro EVB infection nor did it block the killing of EBV-infected targets by cytotoxic T lymphocytes. The cell surface antigen recognized by B532 was shown by immunoprecipitation to have a molecular weight of approximately 45,000.

publication date

  • December 1982

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1073/pnas.79.8.2649

PubMed ID

  • 6283539

Additional Document Info

start page

  • 2649

end page

  • 53

volume

  • 79

number

  • 8 I