DNA methylation, retroviruses, and embryogenesis. Academic Article uri icon

Overview

MeSH

  • Animals
  • Blastocyst
  • Carcinoma
  • Cells, Cultured
  • Embryo, Mammalian
  • Genes, Viral
  • Methylation
  • Mice

MeSH Major

  • DNA, Viral
  • Gene Expression Regulation
  • Moloney murine leukemia virus

abstract

  • By exposing preimplantation embryos to Moloney leukemia virus (M-MuLV), we have previously derived substrains of mice designated as Mov-1-Mov-13 which genetically transmit the virus from one generation to the next. In some of the substrains the inserted viral genome becomes activated at specific stages of embryogenesis and the available evidence suggests that these viral genomes are developmentally regulated. To investigate the effect of cellular differentiation on virus expression, M-MuLV was introduced either into preimplantation or post-implantation mouse embryos or into embryonal carcinoma (EC) cells. Whereas preimplantation embryos or EC cells are not permissive for virus expression, efficient replication occurred in postimplantation embryos or in differentiated cell lines. The viral genomes introduced into early embryonal cells were highly methylated and noninfectious when analyzed in the adult. In contrast, viral genomes introduced into postimplantation embryos or into differentiated cells remained unmethylated and were infectious in a transfection assay. These results demonstrate an efficient de novo methylation activity which appears to be involved in repression of genes introduced into pluripotent embryonal cells and which is not observed in cells of the postimplantation embryo or in differentiated cells in tissue culture.

publication date

  • 1982

has subject area

  • Animals
  • Blastocyst
  • Carcinoma
  • Cells, Cultured
  • DNA, Viral
  • Embryo, Mammalian
  • Gene Expression Regulation
  • Genes, Viral
  • Methylation
  • Mice
  • Moloney murine leukemia virus

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1002/jcb.240200403

PubMed ID

  • 7183678

Additional Document Info

start page

  • 331

end page

  • 336

volume

  • 20

number

  • 4