Enrichment for CFU-C from murine and human bone marrow using soybean agglutinin. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Separation
  • Flow Cytometry
  • Fluorescein-5-isothiocyanate
  • Fluoresceins
  • Humans
  • Mice
  • Species Specificity
  • Thiocyanates

MeSH Major

  • Bone Marrow Cells
  • Hematopoietic Stem Cells
  • Lectins
  • Plant Lectins
  • Soybean Proteins

abstract

  • Mouse bone marrow and spleen cells agglutinated by soybean agglutinin (SBA) or peanut agglutinin (PNA) were previously shown to be enriched for spleen colony-forming cells (CFU-S) and sufficiently depleted of graft-versus-host reaction producing cells to allow hematologic reconstitution of lethally irradiated allogeneic recipient mice. A similar enrichment for cells capable of forming colonies in soft agar culture (CFU-C) has now been found in the SBA-agglutinated fraction of mouse bone marrow cells, in contrast to the finding that in human bone marrow the majority of the CFU-C are in the fraction not agglutinated by SBA. Cytofluorometric studies with fluorescein-labeled SBA (FITC-SBA) revealed that the majority of both mouse and human bone marrow cells bind the lectin. Experiments mixing the human marrow fractions separated by SBA reveal that true enrichment for CFU-C is achieved in the unagglutinated fraction, as opposed to a possible depletion of a suppressor cell population. Granulocytic, monocytic, and mixed cell colonies were all enriched in the SBA-unagglutinated cell fraction from human bone marrow.

publication date

  • February 1982

has subject area

  • Animals
  • Bone Marrow Cells
  • Cell Separation
  • Flow Cytometry
  • Fluorescein-5-isothiocyanate
  • Fluoresceins
  • Hematopoietic Stem Cells
  • Humans
  • Lectins
  • Mice
  • Plant Lectins
  • Soybean Proteins
  • Species Specificity
  • Thiocyanates

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 6799017

Additional Document Info

start page

  • 360

end page

  • 363

volume

  • 59

number

  • 2