α-Actinin and membrane glycoprotein IIIa are different proteins in human blood platelets Academic Article Article uri icon

Overview

MeSH Major

  • Blood Platelets
  • Blood Vessels
  • Purpura
  • Thrombocytopenia

abstract

  • It has been suggested that a platelet protein that is very similar to muscle alpha-actinin is identical to the membrane glycoprotein IIIa (GPIIIa) of platelets and is responsible for anchoring actin filaments directly into the plasma membrane of platelets. To determine if alpha-actinin and GPIIIa are related in platelets, we analyzed the purified proteins on 5% sodium dodecyl sulfate/polyacrylamide gels. The two proteins differ in mobility in both the unreduced and reduced states, and they stain differently with silver stain. In addition, alpha-actinin is a prominent component of the detergent-insoluble cytoskeletons of platelets, whereas GPIIIa is absent from these structures. By using monospecific antisera to the individual proteins, it was demonstrated that alpha-actinin and GPIIIa are immunologically distinct. We conclude that alpha-actinin and GPIIIa are different proteins in human blood platelets and that it is unlikely that alpha-actinin is an integral membrane protein.

publication date

  • September 24, 1982

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1073/pnas.79.2.432

PubMed ID

  • 6176993

Additional Document Info

start page

  • 432

end page

  • 5

volume

  • 79

number

  • 2 I