Human alveolar macrophage growth factor for fibroblasts. Regulation and partial characterization. Academic Article Article uri icon

Overview

MeSH

  • Cell Line
  • Cells, Cultured
  • DNA
  • Humans
  • Macrophage Activation

MeSH Major

  • Fibroblasts
  • Growth Substances
  • Macrophages
  • Peptides
  • Pulmonary Alveoli

abstract

  • The number of fibroblasts composing the alveolar structures in controlled within narrow limits by a strictly modulated rate of fibroblast replication. One possible source of growth-modulating signals for alveolar fibroblasts is the alveolar macrophage, a member of the mononuclear phagocyte family of cells, which collectively are known to be important sources of growth factors for a variety of target cells. To evaluate the role of alveolar macrophages in the control of alveolar fibroblast replication, macrophages from normal individuals obtained by bronchoalveolar lavage were maintained in suspension culture with and without added stimuli, and supernates were evaluated for fibroblast growth-promoting effect. Supernates from unstimulated macrophages contained no growth factor activity. In marked contrast, supernates from macrophages stimulated with particulates and immune complexes contained a growth factor that caused a significant increase in fibroblast replication rate. Maximum growth factor activity was observed 3-4 h after macrophage stimulation, at a concentration of 1-2 x 10(6) macrophages/ml. The alveolar macrophagederived growth factor eluted from DEAE-cellulose at 0.27 M NaCl at neutral pH had an apparent molecular weight of 18,000, and appeared to be distinct from other characterized growth factors. The alveolar macrophage-derived growth factor stimulated lung fibroblast DNA synthesis within 12 h, with cell division apparent within 48 h. In serum-free culture, the alveolar macrophage-derived growth factor by itself did not promote fibroblast replication, but rather acted as a progression factor causing a synergistic increase in fibroblast replication rate in the presence of competence factors such as fibroblast growth factor or platelet-derived growth factor. These studies suggest that when stimulated, human alveolar macrophages may modulate, in part, the replication rate of alveolar fibroblasts by releasing a growth factor within the alveolar microenvironment.

publication date

  • October 1982

has subject area

  • Cell Line
  • Cells, Cultured
  • DNA
  • Fibroblasts
  • Growth Substances
  • Humans
  • Macrophage Activation
  • Macrophages
  • Peptides
  • Pulmonary Alveoli

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC370289

PubMed ID

  • 7119116

Additional Document Info

start page

  • 806

end page

  • 822

volume

  • 70

number

  • 4