Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth. Academic Article uri icon

Overview

MeSH

  • Cell Division
  • Cells, Cultured
  • Female
  • Fetus
  • Fibroblasts
  • Humans
  • Kinetics
  • Nucleic Acid Hybridization
  • Pregnancy
  • Protein Biosynthesis

MeSH Major

  • Lung
  • Procollagen
  • RNA, Messenger

abstract

  • The production of procollagen molecules by human diploid fetal lung fibroblasts (HFL-1 cells) remains constant in both rapid and stationary growth phases. However, log phase cells degrade 3-fold more newly synthesized collagen inside the cell prior to secretion than do stationary phase cells. Procollagen mRNA levels, measured by hybridization with a type I procollagen mRNA-specific complementary DNA, are approximately 2-fold higher in confluent cells than in log phase cells. There are no significant differences in the ability of either log phase or confluent HFL-1 cell procollagen mRNA to be translated in an in vitro cell-free translation system. Therefore, the ability of HFL-1 cells to maintain constant collagen production irrespective of the growth status of the cells results from the combined action of a number of regulatory mechanisms, including changes in procollagen mRNA levels, the utilization of procollagen mRNA, and intracellular procollagen degradation.

publication date

  • March 25, 1981

has subject area

  • Cell Division
  • Cells, Cultured
  • Female
  • Fetus
  • Fibroblasts
  • Humans
  • Kinetics
  • Lung
  • Nucleic Acid Hybridization
  • Pregnancy
  • Procollagen
  • Protein Biosynthesis
  • RNA, Messenger

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 7204395

Additional Document Info

start page

  • 3135

end page

  • 3140

volume

  • 256

number

  • 6