Susceptibility of Entamoeba histolytica to oxygen intermediates Academic Article Article uri icon

Overview

MeSH Major

  • Amphotericin B
  • Antiprotozoal Agents
  • Leishmaniasis, Visceral

abstract

  • To explore the susceptibility of the extracellular protozoan, Entamoeba histolytica, to toxic oxygen intermediates, trophozoites were exposed to fluxes of O2, H2O2, and OH. generated enzymatically by the glucose oxidase and xanthine oxidase reactions. HM-1 trophozoites were resistant to O2, but were readily killed by H2O2 alone. OH. and 1O2 were not required for effective amebicidal activity. The addition of peroxidase and halide enhanced trophozoite killing by H2O2. Sonicates of amebae contained virtually no catalase and little glutathione peroxidase activity which may contribute to susceptibility to H2O2. Coupled with our previous studies with Toxoplasma gondii and Leishmania spp. these observations indicate that there is a broad spectrum of susceptibility of intra- and extracellular pathogenic protozoa to killing by oxygen intermediates.

publication date

  • January 1981

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0166-6851(81)90038-4

PubMed ID

  • 6272108

Additional Document Info

start page

  • 381

end page

  • 91

volume

  • 3

number

  • 6