Phosphorylation of the IgE receptor from ionophore A23187 stimulated intact rat mast cells Academic Article Article uri icon

Overview

MeSH Major

  • Hernia, Obturator
  • Intestinal Obstruction

abstract

  • Purified rat serosal mast cells were labeled either with [32P]orthophosphate or [35S]methionine and their receptors for immunoglobulin E were isolated by repetitive affinity chromatography. In 35S-labeled receptor preparations SDS polyacrylamide gels revealed a broad receptor band, Mr 45,000 to 53,000, and two other bands, Mr 30,000 and 16,000, which apparently represent receptor-associated proteins. Only the receptor band was labeled by 32P. Phosphorylation of receptor was markedly stimulated by the divalent cation ionophore A23187, a known stimulator of histamine release, with changes occuring as early as 15 seconds. This early increase in receptor phosphorylation may be involved in the control of mediator secretion. © 1981.

publication date

  • February 12, 1981

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(81)91184-0

PubMed ID

  • 6452878

Additional Document Info

start page

  • 815

end page

  • 22

volume

  • 98

number

  • 3