Antibody-dependent cell-mediated cytotoxicity for cultured autologous melanoma cells
Signal Processing, Computer-Assisted
Sera from 33 patients with malignant melanoma were tested for antibody-dependent cellmediated cytotoxicity (ADCC) against cultured autologous melanoma cells prelabeled with [3H]proline in a 36-h assay. Effector cells (autologous or from normal donors) were obtained by Ficoll-Hypaque separation of peripheral blood. Assay sensitivity (but not specificity) was affected by the effector cells used, the optimal source of effector cells varying between different autologous serum-target cell combinations. The activity of autologous effector cells appeared to correlate inversely with the extent of disease. Sera from 18 patients gave positive direct tests. In seven cases reactivity was strong enough to permit analysis of specificity with absorption tests. Serum from one patient recognized a melanoma antigen restricted to autologous melanoma cells and three other melanoma cell lines, and not present on other melanoma cell lines or autologous tissues, nonmelanoma tumor cells, or xenogeneic cells. Sera from 5 patients detected a diverse group of widely distributed antigens, all related to fetal calf serum (FCS). Serum from the seventh patient reacted with a widely distributed antigen not related to FCS. © 1981.
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