Structural organization of a 17 KB segment of the alpha 2 collagen gene: evaluation by R loop mapping. Academic Article uri icon

Overview

MeSH

  • Animals
  • Methods
  • Microscopy, Electron
  • Nucleic Acid Hybridization
  • RNA, Messenger
  • Sheep

MeSH Major

  • Collagen
  • DNA, Recombinant
  • Genes

abstract

  • A recombinant phage, SpC3, containing a 17 kb genomic DNA insert representing approximately 60% of the 3' portion of the sheep collagen alpha 2 gene, was evaluated by electron microscopic R loop analysis. A minimum of 17 intervening sequences (introns) and 18 alpha 2 coding sequences (exons) were mapped. With the exception of the 850 base pair exon located at the extreme 3' end of the insert, all exons contained 250 base pairs or less. The total length of all the exons in SpC3 was 3,014 base pairs. The length distribution of the 17 introns ranged from 300 to 1600 base pairs; together, all of the introns comprised 14,070 base pairs of SpC3 DNA. Thus, the DNA region required for coding the interspersed 3 kb of alpha 2 collagen genetic information was 5.6 fold longer than the corresponding alpha 2 mRNA coding sequences.

publication date

  • May 24, 1980

has subject area

  • Animals
  • Collagen
  • DNA, Recombinant
  • Genes
  • Methods
  • Microscopy, Electron
  • Nucleic Acid Hybridization
  • RNA, Messenger
  • Sheep

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC324075

PubMed ID

  • 7433091

Additional Document Info

start page

  • 2241

end page

  • 2253

volume

  • 8

number

  • 10