Endothelial cell adenylate cyclase: activation by catecholamines and prostaglandin I2 Academic Article uri icon

Overview

MeSH Major

  • Adenylyl Cyclases
  • Catecholamines
  • Epoprostenol
  • Muscle, Smooth, Vascular
  • Prostaglandins

abstract

  • Following incubation of intact vascular endothelial cells with 1 mM 3-isobutyl-1-methylxanthine, and isoproterenol or PGI2, cyclic AMP levels increased 4- and 3-fold, respectively. Isoproterenol-stimulated adenylate cyclase activity of cell lysates was selectively inhibited by the β-adrenergic blocking agent propranolol. Catecholamines stimulated adenylate cyclase with the potency series: isoproterenol > epinephrine > norepinephrine. Prostaglandin did not stimulate adenylate cyclase activity in cell lysates, even in the presence of guanine nucleotides or following preincubation of the intact cells with prostaglandins prior to freeze-thaw lysis.

publication date

  • December 1980

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 6255950

Additional Document Info

start page

  • 1640

end page

  • 7

volume

  • 96

number

  • 4