Unreliability of tritiated cholesterol: Studies with [1,2- 3H]cholesterol and [24,25- 3H]cholesterol in humans Academic Article uri icon

Overview

MeSH Major

  • Cholesterol
  • Tritium

abstract

  • Over the past 18 months different lots of [1,2-3H]cholesterol and [24,25-3H]cholesterol were found to be radiochemically acceptable by conventional chemical and other in vitro tests, yet, when co-administered with [4-14C]cholesterol to human subjects, an abrupt fall in the 3H/14C specific activity ratio in plasma cholesterol was discovered in every case. We have concluded that all batches of [3H]cholesterol should be regarded as radiochemically unreliable unless they are shown to behave identically in all respects to [4-14C]cholesterol in an appropriate in vivo assay system.

publication date

  • December 1980

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC348692

PubMed ID

  • 6929548

Additional Document Info

start page

  • 2255

end page

  • 9

volume

  • 77

number

  • 4 I