Immunological properties of subcellular rat lymphocyte preparations. Primary allogeneic stimulation in vitro by fractions containing Ia (RT1-B), but not RT1-A antigens Academic Article uri icon

Overview

MeSH Major

  • Antigens, Surface
  • Isoantigens
  • Lymphocyte Activation
  • Lymphocytes
  • Major Histocompatibility Complex

abstract

  • Rat thymocyte membrane fractions have been prepared which exhibit strain-specific primary mixed-lymphocyte reaction (MLR)-stimulating and Ia (RT1-B) antigenic properties. These preparations lack the antigenicity of classical, serologically-defined RT1-A (Ag-B) antigens, as defined by in vitro serologic assays. Furthermore, after immunization of allogeneic hosts, specific anti-Ia and MLR-blocking antibodies, but not anti-AgB, alloantibodies are elaborated. Thymidine suicide experiments show that the same clones respond to whole cells and the fragments made from those cells, and the response segregates appropriately in F2 progeny as a major histocompatibility complex (RT1)-linked phenomenon. Hence, it is possible to generate Ia-related allogeneic helper signals in primary, as well as secondary, in vitro responses, using subcellular membrane fragments that have restricted expression of RT1-B-, but not RT1-A-, encoded antigens.

publication date

  • July 25, 1980

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2185838

PubMed ID

  • 6445396

Additional Document Info

start page

  • 910

end page

  • 24

volume

  • 151

number

  • 4