Effect of cyclic AMP on the intracellular degradation of newly synthesized collagen. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Line
  • Female
  • Fetus
  • Humans
  • Kinetics
  • Lung
  • Macrophages
  • Pregnancy
  • Rabbits

MeSH Major

  • Cholera Toxin
  • Collagen
  • Cyclic AMP
  • Microbial Collagenase
  • Prostaglandins E

abstract

  • Prostaglandin E1 and cholera toxin increased the intracellular levels of cyclic AMP of human lung fibroblasts. With prostaglandin E1, the increase in cyclic AMP occurred within 10 min followed by a decline to less than one-half of peak values in 6 h. With cholera toxin, the increase occurred within 60 min but the level of cyclic AMP remained increased for 6 h. Both agents caused a decrease in collagen production as expressed as the proportion of newly synthesized protein represented by collagen. The increase in cyclic AMP levels was accompanied by a marked increase in the proportion of newly synthesized collagen which was degraded intracellularly prior to secretion. Analysis of the degraded collagen showed it to be predominantly less than 1000 daltons in molecular mass, but still in peptide linkage. The data are consistent with the hypothesis that cyclic AMP levels in diploid fibroblasts regulate the amount of collagen produced by fibroblasts, at least in part, by modulating the level of intracellular collagen degradation.

publication date

  • April 10, 1980

has subject area

  • Animals
  • Cell Line
  • Cholera Toxin
  • Collagen
  • Cyclic AMP
  • Female
  • Fetus
  • Humans
  • Kinetics
  • Lung
  • Macrophages
  • Microbial Collagenase
  • Pregnancy
  • Prostaglandins E
  • Rabbits

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 6244295

Additional Document Info

start page

  • 2843

end page

  • 2847

volume

  • 255

number

  • 7