Collagenase in the lower respiratory tract of patients with idiopathic pulmonary fibrosis. Academic Article uri icon

Overview

MeSH

  • Adult
  • Aged
  • Body Fluids
  • Bronchi
  • Bronchoscopy
  • Collagen
  • Female
  • Fiber Optic Technology
  • Humans
  • Lymphocytes
  • Male
  • Middle Aged
  • Neutrophils
  • Peptide Hydrolases
  • Pulmonary Alveoli
  • Therapeutic Irrigation
  • Time Factors

MeSH Major

  • Lung
  • Microbial Collagenase
  • Pulmonary Fibrosis

abstract

  • To test the hypothesis that idiopathic pulmonary fibrosis (IPF) is mediated through collagenase present in the lower respiratory tract, we used the fiberoptic bronchoscope to obtain fluid from the lower respiratory tract of 24 patients with IPF, 18 controls and nine patients with sarcoidosis. The fluid was analyzed for a variety of enzymes, including collagenase. Fifteen of 21 patients with IPF showed collagenase activity, whereas normal controls and patients with sarcoidosis showed none (P greater than 0.001, for all comparisons). In two patients with IPF who were re-evaluated after eight to 24 months, the collagenase activity was persistent. Fluid from patients with IPF also contained elevated levels of a non-specific neutral protease (P greater than 0.01 compared with controls), but there was no elastase activity in fluid from patients with IPF or from controls. The collagenase found in lavage fluid in IPF cleaved lung collagen into collagenase-specific TCA and TCB fragments. We conclude that in IPF the collagen of the lung is subjected to sustained lysis, followed by disordered resynthesis, and that the presence of active collagenase in the lower respiratory tract is a specific feature of the alveolitis associated with this disease.

publication date

  • October 4, 1979

has subject area

  • Adult
  • Aged
  • Body Fluids
  • Bronchi
  • Bronchoscopy
  • Collagen
  • Female
  • Fiber Optic Technology
  • Humans
  • Lung
  • Lymphocytes
  • Male
  • Microbial Collagenase
  • Middle Aged
  • Neutrophils
  • Peptide Hydrolases
  • Pulmonary Alveoli
  • Pulmonary Fibrosis
  • Therapeutic Irrigation
  • Time Factors

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1056/NEJM197910043011401

PubMed ID

  • 225666

Additional Document Info

start page

  • 737

end page

  • 742

volume

  • 301

number

  • 14