Gene localization by chromosome fractionation: Globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes Academic Article uri icon

Overview

MeSH Major

  • Chromosomes
  • DNA
  • Estrogens
  • Genes
  • Globins

abstract

  • Chicken metaphase chromosomes were partially purified by rate zonal centrifugation, and DNA was prepared from each of the fractions of the sucrose gradient. The DNA was digested with various restriction enzymes and subjected to electrophoresis in agarose gels. The DNA was transferred to nitrocellulose filters (as described by Southern), and the filters were hybridized with cDNA probes. Four globin genes alpha A, alpha D, beta, and rho or epsilon are located on at least two chromosomes, and three of the estrogen-inducible genes of the hen oviduct--ovalbumin, ovomucoid, and transferrin--are on three different chromosomes. These experiments also confirm our earlier assignment of the endogenous viral sequence related to Rous-associated virus-0 to a separate (and larger) chromosome than the cellular sequence related to the transforming gene of avian sarcoma virus (cellular sarc), although it now appears that cellular sarc is on a small macrochromosome, rather than on a microchromosome.

publication date

  • July 16, 1979

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC383248

PubMed ID

  • 286316

Additional Document Info

start page

  • 1348

end page

  • 52

volume

  • 76

number

  • 3