Inhibition of human seminal fluid DNA polymerase by an IgG fraction of seminal plasma from vasectomized men Academic Article uri icon


MeSH Major

  • Immunoglobulin G
  • Nucleic Acid Synthesis Inhibitors
  • Semen
  • Vasectomy


  • Immunoglobulin G (IgG) was isolated from ejaculates of intact and vasectomized men by precipitation with ammonium sulphate and DEAE-cellulose ionexchange chromatography. Velocity centrifugation revealed that all of the IgG from intact males was 7S protein while less than 40% of the seminal IgG of vasectomized men cosedimented with the 7S marker; the remaining, immunologically unidentifiable, protein was considerably smaller and heterogeneous in size. Only the 7S IgG from the post-vasectomy ejaculates inhibited the activity of a DNA polymerase from the seminal fluid of an intact male. These results suggest that formation of antibody reactive with the seminal fluid DNA polymerase is one manifestation of a vasectomy-associated autoimmune response in man.

publication date

  • December 1978



  • Academic Article



  • eng

PubMed ID

  • 101659

Additional Document Info

start page

  • 97

end page

  • 102


  • 54


  • 1