Expression of collagen biosynthetic activities in lymphocytic cells Academic Article uri icon

Overview

MeSH Major

  • Collagen
  • Lymphocytes
  • Procollagen-Proline Dioxygenase

abstract

  • Immunoglobulin-producing Merwin plasma cells, MPC-11, have been found to contain proplyl hydroxylase (prolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase; EC 1.14.11.2) activity and its crossreacting protein, as well as hydroxyproline and a collagenous protein that could not be classified as type I, II, or III collagen. Friend leukemic cells, on the other hand, contained only prolyl hydroxylase. Thymus-derived (T) lymphocytes and bone-marrow-derived (B) lymphocytes freshly isolated from BALB/c mice expressed low but significant prolyl hydroxylase activity. Upon stimulation with phytohemagglutinin, the enzyme activity in T cells increased 22- to 29-fold. Crossreacting protein was also increased and appeared more stable than the prolyl hydroxylase. The effect of lipopolysaccharide stimulation on B cells uas similar but not as pronounced. Thus, even when not accompanied by other collagen biosynthetic activities, prolyl hydroxylase is present in all cells of hematologic origin.

publication date

  • December 1978

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC411475

PubMed ID

  • 206896

Additional Document Info

start page

  • 1379

end page

  • 83

volume

  • 75

number

  • 3