Release of low density lipoprotein receptors from human fibroblasts or Hela cells by tryptic digestion Academic Article uri icon

Overview

MeSH Major

  • Obesity
  • Thinness
  • Weight Gain

abstract

  • Trypsin treatment of cultured normal human skin fibroblasts or Hela cells releases material which is retained on a low density lipoprotein (LDL)-Sepharose affinity column, may be eluted from it with 2.5 M KI and, after dialysis, agglutinates LDL or apo-B-coated formocells. Such agglutination is prevented by preincubation of the receptor with LDL in solution or with arginine-rich protamine. Trypsin treatment of "receptor defective" or "receptor negative" mutant fibroblasts releases material which is retained on LDL-Sepharose column but fails to agglutinate LDL-coated formocells. The receptor may be labeled with 6-[3H]-glucosamine·HCl and [3H]-leucine, it is inactivated by heating at 80°C for 10 min and may be obtained from normal fibroblasts or Hela cells, whether they were cultured in presence or in absence of lipoprotein-containing fetal calf serum. © 1978.

publication date

  • September 29, 1978

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(78)90179-1

Additional Document Info

start page

  • 366

end page

  • 73

volume

  • 84

number

  • 2