Structure of nascent replicative form DNA of coliphage M13 Academic Article uri icon

Overview

MeSH Major

  • Coliphages
  • DNA Replication
  • DNA, Viral
  • RNA, Viral
  • Virus Replication

abstract

  • Nascent replicative form type II (RFII) DNA of coliphage M13 synthesized in an Escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith DNA polymerase I contains ribonucleotides that are retained in the covalently closed RFI DNA sealed in vitro by the joint action of T5 phage DNA polymerase and T4 phage DNA ligase. These RFI molecules are labile to alkali and RNase H, unlike the RFI produced either in vivo or from RFII with E. coli DNA polymerase I and E. coli DNA ligase. The ribonucleotides are located at one site and predominantly in one strand of the nascent RF DNA. Furthermore, these molecules contain multiple small gaps, randomly located, and one large gap in the intracistronic region.

publication date

  • January 1978

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC411203

PubMed ID

  • 272630

Additional Document Info

start page

  • 153

end page

  • 7

volume

  • 75

number

  • 1