Isolation of a nuclear DNA synthesizing complex from human sperm Academic Article Article uri icon

Overview

MeSH Major

  • Cultural Diversity
  • Education, Medical, Undergraduate
  • Education, Professional
  • Minority Groups
  • Program Evaluation

abstract

  • A simplified procedure is described for isolating a human sperm nuclear DNA-synthesizing complex which does not involve the use of detergent or necessitate the prior purification of membrane-free nuclei. Selective disruption of sperm heads is accomplished by the use of dithiothreitol, trypsin, and deoxyribonuclease. The particulate complex is freed of soluble components by sedimentation through a solution of 19% (w/v) Metrizamide and further purified by buoyant density centrifugation. The characteristics of this endogenously templated nuclear DNA polymerase differ from other sperm-associated polymerases that have been reported. © 1977 Academic Press, Inc. All rights of reproduction in any form reserved.

publication date

  • August 22, 1977

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/S0006-291X(77)80135-6

PubMed ID

  • 901540

Additional Document Info

start page

  • 1404

end page

  • 10

volume

  • 77

number

  • 4