Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13 Academic Article Article uri icon

Overview

MeSH Major

  • Arthroplasty, Replacement, Knee
  • Knee Prosthesis
  • Prosthesis-Related Infections

abstract

  • With precise conditions of digestion with single-strand-specific nucleases, namely, endonuclease S1 of Aspergillus oryzae and exonuclease I of Escherichia coli, nuclease-resistant DNA cores can be obtained reproducibly from single-stranded M13 DNA. The DNA cores are composed almost exclusively of two sizes (60 and 44 nucleotides long). These have high (G + C)-contents relative to that of intact M13 DNA, and arise from restricted regions of the M13 genome. The resistance of these fragments to single-strand-specific nucleases and their nondenaturability strongly suggest the presence of double-stranded segments in these core pieces. That the core pieces are only partially double-stranded is shown by their lack of complete base complementarity and their pattern of elution from hydroxyapatite. © 1977.

publication date

  • December 21, 1977

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(77)91109-3

PubMed ID

  • 603641

Additional Document Info

start page

  • 1037

end page

  • 44

volume

  • 79

number

  • 4