DNA synthesizing activity in normal human sperm. Location and characterization of the endogenous reaction
Genome-Wide Association Study
Nucleic Acid Amplification Techniques
Chromatin-associated complexes have been purified from membrane-free nuclei of normal human sperm heads. They band at a sucrose buoyant density of 1.15-1.19 g/ml and contain macromolecular components which catalyze the endogenous synthesis of DNA in a reaction partially sensitive to ribonuclease pretreatment. The sedimentation rate and buoyant density of the purified reaction product, still attached to its endogenous template, demonstrate that the [3H]DNA is associated with a high molecular weight RNA, which may be serving as both template and primer. © 1977.
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