DNA synthesizing activity in normal human sperm. Location and characterization of the endogenous reaction Academic Article Article uri icon

Overview

MeSH Major

  • DNA
  • Genome-Wide Association Study
  • Nucleic Acid Amplification Techniques
  • Premature Birth
  • Specimen Handling

abstract

  • Chromatin-associated complexes have been purified from membrane-free nuclei of normal human sperm heads. They band at a sucrose buoyant density of 1.15-1.19 g/ml and contain macromolecular components which catalyze the endogenous synthesis of DNA in a reaction partially sensitive to ribonuclease pretreatment. The sedimentation rate and buoyant density of the purified reaction product, still attached to its endogenous template, demonstrate that the [3H]DNA is associated with a high molecular weight RNA, which may be serving as both template and primer. © 1977.

publication date

  • January 1977

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0014-4827(77)90239-7

PubMed ID

  • 852518

Additional Document Info

start page

  • 47

end page

  • 54

volume

  • 106

number

  • 1