A particulate DNA polymerase activity in adult rat brain Academic Article Article uri icon

Overview

MeSH Major

  • Cultural Diversity
  • Education, Medical, Undergraduate
  • Education, Professional
  • Minority Groups
  • Program Evaluation

abstract

  • A particulate fraction of adult rat brain (sucrose buoyant density 1.24 gm/ml) catalyzed the incorporation of [3H]dTTP into an acid-insoluble product in an endogenously templated reaction sensitive to ribonuclease pretreatment. Upon fractionation, this activity was identified in the cerebellum, pons, frontal lobes and base. The DNA polymerase present in these brain fractions exhibited a strong preference for the synthetic template dT12-18·poly rA rather than dT12-18·poly dA; dT10 was completely inactive. Purification and equilibrium Cs2SO4 gradient centrifugation of the [3H]DNA product-endogenous template complex suggested that RNA was serving as primer for endogenous DNA synthesis. © 1977.

publication date

  • April 11, 1977

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(77)91510-8

PubMed ID

  • 856178

Additional Document Info

start page

  • 568

end page

  • 75

volume

  • 75

number

  • 3