Dopamine synthesis in rat brain striatal synaptosomes. II. Dibutyryl cyclic adenosine 3':5' monophosphoric acid and 6 methyltetrahydropterine induced synthesis increases without an increase in endogenous dopamine release Academic Article uri icon

Overview

MeSH Major

  • Bucladesine
  • Corpus Striatum
  • Dopamine
  • Pterins
  • Synaptosomes

abstract

  • The effects of N6, O2'-dibutyryl cyclic adenosine 3':5'-monophosphoric acid (dibutyryl cyclic AMP), DL-6-methyl-5, 6, 7, 8-tetrahydropterine (6-MPH4) and tyramine on dopamine synthesis and endogenous dopamine release in rat brain striatal synaptosomes have been examined. Dibutyryl cyclic AMP (1 mM) produced a 100% increase in dopamine synthesis but had no effect on endogenous dopamine release. Veratridine (75 muM) did not further stimulate dibutyryl cyclic AMP-treated tissue. 6-MPH4 (0.1 mM) also stimulated synthesis without stimulating dopamine release. The stimulation produced by 6-MPH4 was additive to the stimulations produced by either dibutyryl cyclic AMP or veratridine. Tyramine (10(-4) M) inhibited synthesis, while increasing dopamine release. These data suggest that 1) dopamine-releasing agents may have differential effects upon dopamine synthesis, depending on their mechanism of release, 2) dopamine synthesis can be stimulated independently of endogenous dopamine release and 3) the pteridine cofactor necessary for tyrosine hydroxylation is not normally present at saturating levels.

publication date

  • December 1976

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 177758

Additional Document Info

start page

  • 97

end page

  • 104

volume

  • 197

number

  • 1