The solid phase synthesis of a protein activator for lecithin cholesterol acyltransferase corresponding to human plasma apoC I Academic Article Article uri icon


MeSH Major

  • Lipoproteins, HDL


  • Apolipoprotein C-I, a protein constituent of the very low density lipoproteins of human plasma, consists of a single chain of 57 amino acids. The total synthesis of a protein corresponding to apolipoprotein C-I in physical properties and compositions was accomplished by solid phase techniques employing a modified polystrene incorporating spacer groups between the point of attachment of the first residue and the polymer matrix. The synthetic apoprotein was shown to activate lecithin:cholesterol acyltransferase to the same extent as the native protein. Comparative lipid-binding studies with dimyristoyl phosphatidylcholine gave complexes for native and synthetic apoprotein which floated at the same density after ultracentrifugation in KBr gradients and had virtually the same lipid:protein ratios.

publication date

  • December 1976



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1073/pnas.73.5.1422

PubMed ID

  • 179085

Additional Document Info

start page

  • 1422

end page

  • 6


  • 73


  • 5