Subunit structure of factor VIII antigen synthesized by cultured human endothelial cells Academic Article uri icon

Overview

MeSH Major

  • Antigens
  • Endothelium
  • Factor VIII

abstract

  • Cultured human endothelial cells were labeled with (3H)leucine, and the radioactive Factor VIII antigen present in the postculture medium was isolated by double anitbody immunoprecipitation and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol. The Factor VIII antigen synthesized by cultured endothelial cells was found to contain the same single polypeptide subunit (mol wt 225,000) present in plasma Factor VIII antigen. These results suggest that in vivo, the endothelial cell is a major site of synthesis of circulating Factor VIII antigen.

publication date

  • December 1975

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC301918

PubMed ID

  • 1159083

Additional Document Info

start page

  • 698

end page

  • 702

volume

  • 56

number

  • 3