Paper electrophoresis and chromatography of oligopeptides with N-terminal methionine Academic Article uri icon

Overview

MeSH Major

  • Braces
  • Exercise Therapy
  • Intervertebral Disc
  • Low Back Pain
  • Spinal Diseases

abstract

  • Several electrophoretic and chromatographic systems are described for the separation of di- and tripeptides with N-terminal methionine. The most effective methods of separation are electrophoresis in barbital-triethylamine-acetic acid, pH 8.0 and chromatography in n-butanol-acetic acid-water, 18:2:5. Increased resolution may be obtained by using two or more systems in combination. These methods were intended to separate and identify the oligopeptides formed during the initiation of eukaryotic protein synthesis, but may also be applied to the separation of small peptides generally. © 1973.

publication date

  • January 1973

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(73)90259-5

Additional Document Info

start page

  • 161

end page

  • 9

volume

  • 54

number

  • 1