Rapid axonal transport of [3H]fucosyl glycoproteins in the goldfish optic system
After an intraocular injection of [3H]fucose, labeled glycoproteins are rapidly transported to the goldfish optic tectum at a rate of 70-80 mm/day at 21 °C. A small amount of TCA-soluble metarial is also transported. Slow transport of labeled glycoproteins was not detected. Most of the transported glycoproteins are bound to sedimentable subcellular particles. Synthesis of the transported glycoproteins is prevented when retinal protein synthesis is inhibited with acetyxycycholheximide. These findings agree with the results of previous studies with labeled glucosamine, which suggested that carbohydrates are incorporated into glycoproteins in the nerve cell body and then transported to the axons and endings. [3H]Fucose produces a relatively long-lived pool of labeled precursor in the retina; more than half of the transported labeled glycoprotein in the tectum 24 h after a [3H]fucose injection is synthesized later than 6 h after the injection. © 1972.
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