Denaturation of DNA after X-irradiation in vitro
Arthroplasty, Replacement, Knee
X-irradiation of E. coli DNA caused partial strand-separation as judged by susceptibility of the DNA to nuclease specific for single-stranded DNA, namely an endonuclease from Neurospora crassa and exonuclease I of E. coli. The elution profile of X-irradiated DNA from hydroxyapatite column showed that single-stranded fragments were produced. These fragments had a sedimentation coefficient of 3-4s corresponding to a length of about 250 nucleotides. © 1970.
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