Denaturation of DNA after X-irradiation in vitro Academic Article Article uri icon

Overview

MeSH Major

  • Arthroplasty, Replacement, Knee
  • Knee Prosthesis
  • Prosthesis-Related Infections

abstract

  • X-irradiation of E. coli DNA caused partial strand-separation as judged by susceptibility of the DNA to nuclease specific for single-stranded DNA, namely an endonuclease from Neurospora crassa and exonuclease I of E. coli. The elution profile of X-irradiated DNA from hydroxyapatite column showed that single-stranded fragments were produced. These fragments had a sedimentation coefficient of 3-4s corresponding to a length of about 250 nucleotides. © 1970.

publication date

  • August 24, 1970

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(70)90972-1

PubMed ID

  • 4924670

Additional Document Info

start page

  • 793

end page

  • 9

volume

  • 40

number

  • 4