L‐asparaginase activity in plasma and other biological fluids Academic Article Article uri icon

Overview

MeSH Major

  • Heart Ventricles
  • Purkinje Fibers
  • Signal Processing, Computer-Assisted

abstract

  • An automated method has been developed for the assay of L‐asparaginase activity in plasma and other biological fluids. By means of this method, L‐asparaginase activity has been determined in plasma, cerebrospinal fluid, and urine in patients receiving E. coli L‐asparaginase (EC‐2) therapy. The height of the plasma activity was related to the dosage. In some patients, the plasma enzyme activity fell despite continued administration of L‐asparaginase. The disappearance of the E. coli L‐asparaginase from the plasma was related to the commercial source of the enzyme. From one source the half‐life in adults was 11.2 ± 2.7 hours and from another, 22.6 ± 4.7 hours. Intramuscular administration of enzyme resulted in plasma levels that were about half those observed following intravenous administration of enzyme. Following intravenous administration of L‐asparaginase at doses up to 5000 IU/kg/day, no activity was found in urine. At the highest dosage levels, small amounts of activity were observed in cerebrospinal fluid. When enzyme was injected directly into the cerebrospinal fluid, there was a rapid transfer of enzyme to the plasma, and the activity in the spinal fluid was reduced to negligible amounts within 24 hours. Copyright © 1970 American Cancer Society

publication date

  • January 1970

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1002/1097-0142(197002)25:2<244::AID-CNCR2820250203>3.0.CO;2-V

PubMed ID

  • 4905152

Additional Document Info

start page

  • 244

end page

  • 52

volume

  • 25

number

  • 2