PYRIMIDINE SYNTHESIS IN TISSUE CULTURE Academic Article Article uri icon

Overview

MeSH Major

  • Carcinoma, Transitional Cell
  • Ureteral Neoplasms
  • Ureteral Obstruction

abstract

  • Abstract— Myelinated cerebellar tissue culture (organ culture) was used to assess the salvage and de novo pathways of pyrimidine synthesis in mammalian brain. Radioactive orotic acid and carbamyl aspartic acid were readily incorporated into UMP and into perchloric acid‐insoluble RNA. The incorporation was effectively blocked by azauridine. Neither radioactive sodium bicarbonate or citrulline was incorporated into UMP or blocked by azauridine. [3H]Uridine, on the other hand, rapidly entered the cultures, was incorporated into UMP and perchloric acid‐insoluble material, and was partially inhibited by azauridine. The failure to demonstrate activity of carbamyl phosphate synthetase suggests the potential importance of the salvage pathway and the likely dependence of the brain upon exogenous and endogenous pyrimidine precursors. Copyright © 1968, Wiley Blackwell. All rights reserved

publication date

  • January 1968

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1111/j.1471-4159.1968.tb05925.x

PubMed ID

  • 5729423

Additional Document Info

start page

  • 1437

end page

  • 43

volume

  • 15

number

  • 12