Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglyercol Academic Article uri icon

Overview

MeSH Major

  • Avian Sarcoma Viruses
  • Cell Transformation, Neoplastic
  • Diglycerides
  • Genes
  • Genes, Viral
  • Glycerides
  • Phosphatidylinositols
  • Protein Kinases
  • Viral Proteins

abstract

  • The ability of purified Rous sarcoma virus transforming gene product, pp60v-src, to phosphorylate phosphatidylinositol and diacylglycerol was investigated. Phosphatidylinositol was phosphorylated to form both mono- and diphosphorylated derivatives. 1,2-Diacylglycerol was phosphorylated to form phosphatidic acid. These activities showed the same thermolability and the same sensitivity to inhibitors as shown by the casein kinase activity of pp60v-src. In addition, when serum-starved chicken embryo fibroblasts transformed by a virus mutant temperature-sensitive for transformation were shifted from the nonpermissive to permissive temperature, an increase of 50-100% in the labeling of phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate, and phosphatidic acid was observed, as compared to uninfected cells.

publication date

  • January 1984

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC345448

PubMed ID

  • 6326105

Additional Document Info

start page

  • 2117

end page

  • 21

volume

  • 81

number

  • 7 I