Cell-surface glycoproteins of human sarcomas: Differential expression in normal and malignant tissues and cultured cells Academic Article uri icon

Overview

MeSH Major

  • Membrane Glycoproteins
  • Sarcoma

abstract

  • Normal differentiation and malignant transformation of human cells are characterized by specific changes in surface antigen phenotype. In the present study, we have defined six cell-surface antigens of human sarcomas and normal mesenchymal cells, by using mixed hemadsorption assays and immunochemical methods for the analysis of cultured cells and immunohistochemical staining for the analysis of normal tissues and greater than 200 tumor specimens. Differential patterns of F19 (Mr, 120,000/95,000 glycoprotein), F24 (Mr, 95,000 glycoprotein), G171 (Mr, 75,000 glycoprotein), G253 (Mr, 90,000 glycoprotein), S5 (Mr, 120,000 glycoprotein), and Thy-1 (Mr, 25,000 glycoprotein) antigen expression were found to characterize (i) subsets of cultured sarcoma cell lines, (ii) cultured fibroblasts derived from various organs, (iii) normal resting and activated mesenchymal tissues, and (iv) sarcoma and nonmesenchymal tumor tissues. These results provide a basic surface antigenic map for cultured mesenchymal cells and mesenchymal tissues and permit the classification of human sarcomas according to their antigenic phenotypes.

publication date

  • January 1988

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC280153

PubMed ID

  • 2896356

Additional Document Info

start page

  • 3110

end page

  • 4

volume

  • 85

number

  • 9