Transforming growth factor beta1 rescues serum deprivation-induced apoptosis via the mitogen-activated protein kinase (MAPK) pathway in macrophages. Academic Article uri icon

Overview

MeSH

  • Animals
  • Base Sequence
  • Cell Line
  • Culture Media, Serum-Free
  • DNA Primers
  • Mice
  • Protein-Serine-Threonine Kinases
  • Receptors, Transforming Growth Factor beta
  • Signal Transduction

MeSH Major

  • Apoptosis
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Macrophages
  • Transforming Growth Factor beta

abstract

  • Cell death and cell survival are central components of normal development and pathologic states. Transforming growth factor beta1 (TGF-beta1) is a pleiotropic cytokine that regulates both cell growth and cell death. To better understand the molecular mechanisms that control cell death or survival, we investigated the role of TGF-beta1 in the apoptotic process by dominant-negative inhibition of both TGF-beta1 and mitogen-activated protein kinase (MAPK) signaling pathways. Murine macrophages (RAW 264.7) undergo apoptosis following serum deprivation, as determined by DNA laddering assay. However, apoptosis is prevented in serum-deprived macrophages by the presence of exogenous TGF-beta1. Using stably transfected RAW 264.7 cells with the kinase-deleted dominant-negative mutant of TbetaR-II (TbetaR-IIM) cDNA, we demonstrate that this protective effect by TGF-beta1 is completely abrogated. To determine the downstream signaling pathways, we examined TGF-beta1 effects on the MAPK pathway. We show that TGF-beta1 induces the extracellular signal-regulated kinase (ERK) activity in a time-dependent manner up to 4 h after stimulation. Furthermore, TGF-beta1 does not rescue serum deprivation-induced apoptosis in RAW 264.7 cells transfected with a dominant-negative mutant MAPK (ERK2) cDNA or in wild type RAW 264.7 cells in the presence of the MAPK kinase (MEK1) inhibitor. Taken together, our data demonstrate for the first time that TGF-beta1 is an inhibitor of apoptosis in cultured macrophages and may serve as a cell survival factor via TbetaR-II-mediated signaling and downstream intracellular MAPK signaling pathway.

publication date

  • April 16, 1999

has subject area

  • Animals
  • Apoptosis
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Cell Line
  • Culture Media, Serum-Free
  • DNA Primers
  • Macrophages
  • Mice
  • Protein-Serine-Threonine Kinases
  • Receptors, Transforming Growth Factor beta
  • Signal Transduction
  • Transforming Growth Factor beta

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 10196228

Additional Document Info

start page

  • 11362

end page

  • 11368

volume

  • 274

number

  • 16